On the path of swordsmanship in a "Futuristic" yet Feudal Japan, it is said that the one who becomes No.1 will rule the world. But the only rule in this world is that only the No. 2 is allowed to fight the No. 1. The Afro Samurai who is the current No. 2, travels the road looking for revenge on the man who killed his father, a three-armed gunman who has harnessed god-like power and is the current No. 1.